Instructions:

1. File Format: All files are provided as tab-delimited text files (.txt).
2. Finding Your Kit or Plate: Use the search function in your browser to quickly locate your specific kit or plate by part number.
   • On Windows: Press Ctrl + F
   • On Mac: Press Cmd + F
   • Enter the kit or plate number in the search bar that appears and press Enter.
3. Downloading the Barcode Sequence File:
   • Once you find the link to the barcode sequence file you need, right-click on the link.
   • Select "Save link as..." or "Save as..." from the context menu.
   • Choose a location on your device to save the file and click "Save".
4. File Organization: Files are organized into three columns.
   • Column 1: Sample/Index ID
   • Column 2: Index 1 (i7)
   • Column 3: Index 2 (i5)
5. Sequencer Input/Setup:
   • For some Illumina instruments, you may need to reverse complement the Index 2 (i5) sequences. Please review the information here to determine the correct orientation for your particular sequencer.
   • We recommend using this reverse complement tool to perform reverse complementing.

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MagicPrep NGS kits:

Product name	Kit number	Component number	Index (barcode) files
Revelo DNA-Seq Enz for MagicPrep NGS, kit A	30186624	30188830 30188831 30188832 30188833	MagicPrepUDI_A1 MagicPrepUDI_A2 MagicPrepUDI_A3 MagicPrepUDI_A4
Revelo DNA-Seq Enz for MagicPrep NGS, kit B	30186625	30188844 30188845 30188846 30188847	MagicPrepUDI_B1 MagicPrepUDI_B2 MagicPrepUDI_B3 MagicPrepUDI_B4
Revelo DNA-Seq Enz for MagicPrep NGS, kit C	30186626	30188852 30188853 30188854 30188855	MagicPrepUDI_C1 MagicPrepUDI_C2 MagicPrepUDI_C3 MagicPrepUDI_C4
Revelo DNA-Seq Mech for MagicPrep NGS, kit A	30186627	30188830 30188831 30188832 30188833	MagicPrepUDI_A1 MagicPrepUDI_A2 MagicPrepUDI_A3 MagicPrepUDI_A4
Revelo DNA-Seq Mech for MagicPrep NGS, kit B	30186628	30188844 30188845 30188846 30188847	MagicPrepUDI_B1 MagicPrepUDI_B2 MagicPrepUDI_B3 MagicPrepUDI_B4
Revelo DNA-Seq Mech for MagicPrep NGS, kit C	30186629	30188852 30188853 30188854 30188855	MagicPrepUDI_C1 MagicPrepUDI_C2 MagicPrepUDI_C3 MagicPrepUDI_C4
Revelo mRNA-Seq for MagicPrep NGS, kit A	30186621	30188830 30188831 30188832 30188833	MagicPrepUDI_A1 MagicPrepUDI_A2 MagicPrepUDI_A3 MagicPrepUDI_A4
Revelo mRNA-Seq for MagicPrep NGS, kit B	30186622	30188844 30188845 30188846 30188847	MagicPrepUDI_B1 MagicPrepUDI_B2 MagicPrepUDI_B3 MagicPrepUDI_B4
Revelo mRNA-Seq for MagicPrep NGS, kit C	30186623	30188852 30188853 30188854 30188855	MagicPrepUDI_C1 MagicPrepUDI_C2 MagicPrepUDI_C3 MagicPrepUDI_C4
All MagicPrep kits	All 96 indexing UDI pairs in one file		MagicPrepUDI_all

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DNA-Seq kits:

Product name	Kit number	Component number	Index (barcode) files
Celero DNA-Seq Mech Library Preparation	30238473 30188861	30190759 S02702 S02709 S02710 30238474 30219997 30219998 30219999 30220000	TecanUDI_A_8nt TecanUDI_B_8nt TecanUDI_C_8nt TecanUDI_D_8nt TecanUDI_A_10nt_24 TecanUDI_A_10nt TecanUDI_B_10nt TecanUDI_C_10nt TecanUDI_D_10nt
Celero DNA-Seq Enz Library Preparation	30238472 30188860	30190759 S02702 S02709 S02710 30238474 30219997 30219998 30219999 30220000	TecanUDI_A_8nt TecanUDI_B_8nt TecanUDI_C_8nt TecanUDI_D_8nt TecanUDI_A_10nt_24 TecanUDI_A_10nt TecanUDI_B_10nt TecanUDI_C_10nt TecanUDI_D_10nt
Celero DNA-Seq (legacy kit)	0360-xx 0360x-96 0360x-UDI	S02521-S02528 S02529 S02530 S02531 S02532 S02533 S02534 S02702 S02709 S02710	TecanBC_I_1-8 Metaplex_A_1-24 Metaplex_A Metaplex_B Metaplex_C Metaplex_D TecanUDI_A_8nt TecanUDI_B_8nt TecanUDI_C_8nt TecanUDI_D_8nt
Celero EZ DNA-Seq (legacy kit)	9363-xx 9363x-xxx 0568-24 0569-24 0568x-A01 0569x-A01	S02529 S02695 S02530 S02531 S02532 S02533 S02534 S02702 S02709 S02710	Metaplex_A_1-24 TecanUDI_A_1-24 Metaplex_A Metaplex_B Metaplex_C Metaplex_D TecanUDI_A_8nt TecanUDI_B_8nt TecanUDI_C_8nt TecanUDI_D_8nt
Ovation Ultralow System V2 DNA-Seq	0344NB-08 0344NB-32 0344-32 0344NB-A01 9149-A01	S02309-S02316 S02317 S02366, S02366A S02215	TecanBC_I_1-8 TecanBC_I_1-32 TecanBC_I TecanUDI_A_8nt

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RNA-Seq kits:

Product name	Kit number	Component number	Index (barcode) files
Revelo RNA-Seq High Sensitivity	30184147 30184149 30184151 30184204	S02309-S02316 S02317 S02477 30184203	TecanBC_I_1-8 TecanBC_I_1-32 TecanBC_I TecanUDI_A_8nt
Universal Plus Total RNA-Seq	915x-24 915x-A01 915xB-A01	S02622 S02480 S02690 30185200 30185201	TecanUDI_A_8nt_1-24 TecanUDI_A_8nt TecanUDI_B_8nt TecanUDI_C_8nt TecanUDI_D_8nt
Universal Plus mRNA-Seq	052x-24 052x-A01 052xB-A01	S02622 S02480 S02690 30185200 30185201	TecanUDI_A_8nt_1-24 TecanUDI_A_8nt TecanUDI_B_8nt TecanUDI_C_8nt TecanUDI_D_8nt
Ovation SoLo RNA-Seq	0500-xx 0501-xx 0502-xx	S02221 S02238, S02574	TecanBC_I_1-32 TecanBC_I
Universal Prokaryotic RNA-Seq	0363-32	S02511 S02480	TecanBC_III_1-32 TecanUDI_A_8nt
Universal RNA-Seq	053x-32 053x-A01 CUN-003	S02511 S01989 S02480 S02690	TecanBC_III_1-32 TecanBC_III TecanUDI_A_8nt TecanUDI_B_8nt

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Methyl-Seq kits:

Product name	Kit number	Component number	Index (barcode) files
Ovation RRBS Methyl-Seq with TrueMethyl oxBS	0553-32 9522-A01	S02140-S02155 S02223	TecanRRBS_1-16 TecanBC_I
Ultralow Methyl-Seq with TrueMethyl oxBS	0541-32 9513-A01	S02694 S02169	TecanBC_I_1-32 TecanBC_III

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Target Enrichment kits:

Product name	Kit number	Component number	Index (barcode) files
Allegro Targeted Genotyping V2	1005x-xxx 0413-16	S02684 S02685 S02435-S02450	TecanBC_I TecanBC_II AllegroMetaplex